Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados

María Mercedes Zambrano1, Carlos Ruiz1, Ivan Camilo Acosta1, SiB Colombia2, Angela Cantillo3, Corporación Corpogen4

1 Corporación Corpogen, Carrera 5 #66A-34, 110231, Bogotá, Colombia; 2 ; 3 Corporación Corpogen, Bogotá, Colombia; 4 Corporación Corpogen, Carrera 5 #66A-34, 110231, Bogotá, Colombia

Corresponding author(s): María Mercedes Zambrano (mzambrano@corpogen.org)Carlos RuizIvan Camilo Acosta, SiB Colombia (sib@humboldt.org.co), Angela Cantillo

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Zambrano M M, Ruiz C, Acosta I C (2020): Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados. v1.5. Corporación CorpoGen. Dataset/Occurrence. https://doi.org/10.15472/avygwj http://doi.org/10.15472/avygwj

Abstract

These microorganisms were collected from the phyllosphere of Espeletia plants located in paramo ecosystems in the National Natural Park Los Nevados. The work was part of the Colombian Center of Genomics and Bioinformatics, GEBIX, a research consortium than worked on metagenomics and bioprospecting from 2008 to 2014.

Keywords: Phyllosphere microbiome, Espeletia phyllosphere, Occurrence, Observation

General description

Purpose: These strains were obtaine to have a collection of microorganisms from plants in the páramo ecosystems in order to study possible adaptations to harsh environmental conditions such as resistance to UV and daily temperature changes.

Project details

Project title: Bioprospección, bioinformática y metagenómica en el Parque Nacional Natural los Nevados
Personnel: María Mercedes Zambrano, Carlos Ruiz, Ivan Camilo Acosta
Funding: Colciencias
Universidad y Centros participantes
Study area descriptions/descriptor: Espeletia plant phyllosphere
Design description: The idea was to analyze microbial communities associated with endemic Espeletia plants. Communities were analyzed by massive sequencing (Ruiz-Pérez, 2016) and isolates were obtained to identify properties that enable microbial survival in these locations.

Data published through GBIF: https://ipt.biodiversidad.co/sib/resource?r=filosfera

Taxonomic coverage

General taxonomic coverage description: Microorganismos pertencientes a Bacteria y Eukarya, en la mayoría a nivel de género.
Taxonomic ranks
Domain: Bacteria, Eukarya

Spatial coverage

General spatial coverage: The samples were collected at a specific location within the PNN los Nevados (04°52'27'' N  75°15'51.4'' W). This park is located on the Cordillera Central in Colombia, it has altitudes between 2,600 and 5,321 m.a.s.l., and an area of approximately 58,300 hectares with diverse ecosystems (Van Wyngaarden, W., & Fandiño, M. 2002). It is located within the Departments of Caldas (Municipio de Villamaría), Risaralda (Municipios de Santa Rosa de Cabal y Pereira), Quindío (Municipio de Salento) and Tolima (Municipios de Ibagué, Anzoátegui, Santa Isabel, Murillo, Villahermosa, Casabianca y Herveo)(www.parquesnacionales.gov.co/).
Coordinates: 4°52'26.4''N and 4°52'26.4''N Latitude; 75°16'12''W and 75°16'12''W Longitude 

Temporal coverage: April 12, 2013

Natural collections description

Parent collection identifier: CG
Collection name: Cepario Corpogen
Collection identifier: Registro Nacional de Colecciones Biológicas:140
Specimen preservation method: Deep frozen

Methods

Method step description: Microbes were recovered from leaf surfaces by plating on solid R2A Agar (Difco) and Tryptone soy agar (TSA, Oxoid), and incubating several days at 25ºC. Strains were purified by re-streaking, characterized by growth on various media, and checked for the ability to withstand UVC and temperature changes. Strains were identified by sequence analysis of either the 16S rRNA gene or the ITS region.
Study extent description: Microbial isolates were obtained from Espeletia leaves sampled from plants located in páramo ecosystems (04°52'27'' N  75°15'51.4'' W).
Sampling description: Leaf samples from various plant parts were taken, in triplicate, and transported to the lab where they were processed to recover culturable microorganisms and DNA.

Datasets

Dataset description
Object name: Darwin Core Archive Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: https://ipt.biodiversidad.co/sib/archive.do?r=filosfera
Publication date of data: 2020-11-19
Language: English
Licences of use: Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License

Metadata language: English
Date of metadata creation: 2018-02-01
Hierarchy level: Dataset

References
Ruiz-Pérez CA, Restrepo S, Zambrano MM. 2016. Microbial and functional
diversity within the phyllosphere of Espeletia species in an Andean high-mountain
ecosystem. Appl Environ Microbiol 82:1807–1817 doi:10.1128/AEM.02781-15
Van Wyngaarden, W., & Fandiño, M. (2002) Parque Nacional los Nevados. Un caso de selección y zonificación de áreas de conservación biológica. In Serie Investigación 4. Javergraf (ed.) Bogotá - Colombia: Pontificia Universidad Javeriana.