Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados

最新版本 由 Corporación CorpoGen 發佈於 Aug 1, 2019 Corporación CorpoGen

These microorganisms were collected from the phyllosphere of Espeletia plants located in paramo ecosystems in the National Natural Park Los Nevados. The work was part of the Colombian Center of Genomics and Bioinformatics, GEBIX, a research consortium than worked on metagenomics and bioprospecting from 2008 to 2014.

Data Records

The data in this 出現紀錄 resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 50 records. 1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.

  • Occurrence (核心)
    50
  • MeasurementOrFact 
    250

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

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版本

The table below shows only published versions of the resource that are publicly accessible.

如何引用

研究者應依照以下指示引用此資源。:

Zambrano M M, Ruiz C, Acosta I C (2018): Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados. v1.4. Corporación CorpoGen. Dataset/Occurrence. https://doi.org/10.15472/avygwj

Rights

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The publisher and rights holder of this work is Corporación CorpoGen. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: 9a6c17ba-58f6-4186-83a1-7ca3386deb73。  Corporación CorpoGen 發佈此資源,並經由Colombian Biodiversity Information System同意向GBIF註冊成為資料發佈者。

關鍵字

Phyllosphere microbiome; Espeletia phyllosphere; Occurrence; Observation

聯絡資訊

資源建立者:

María Mercedes Zambrano
Directora Científica
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org
Carlos Ruiz
Investigador
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org
Ivan Camilo Acosta
Investigador
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org

可回覆此資源相關問題者:

María Mercedes Zambrano
Directora Científica
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org

元數據填寫者:

SiB Colombia
María Mercedes Zambrano
Directora Científica
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org
Angela Cantillo
Investigadora
Corporación Corpogen Bogotá CO

與此資源的相關者:

作者
Corporación Corpogen
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá CO 18050122

地理涵蓋範圍

The samples were collected at a specific location within the PNN los Nevados (04°52'27'' N 75°15'51.4'' W). This park is located on the Cordillera Central in Colombia, it has altitudes between 2,600 and 5,321 m.a.s.l., and an area of approximately 58,300 hectares with diverse ecosystems (Van Wyngaarden, W., & Fandiño, M. 2002). It is located within the Departments of Caldas (Municipio de Villamaría), Risaralda (Municipios de Santa Rosa de Cabal y Pereira), Quindío (Municipio de Salento) and Tolima (Municipios de Ibagué, Anzoátegui, Santa Isabel, Murillo, Villahermosa, Casabianca y Herveo)(www.parquesnacionales.gov.co/).

界定座標範圍 緯度南界 經度西界 [4.874, -75.27], 緯度北界 經度東界 [4.874, -75.27]

分類群涵蓋範圍

Microorganismos pertencientes a Bacteria y Eukarya, en la mayoría a nivel de género.

Domain  Bacteria,  Eukarya

時間涵蓋範圍

起始日期 2013-04-12

計畫資料

The Colombian Center for Genomics and Bioinformatics of Extreme Environments (GeBiX) was established in 2008 and carried out research until 2014. This Center worked as a network that involved 14 research groups in 7 national institutions (Universidad Nacional de Colombia - Bogotá, Universidad del Cauca, Universidad del Valle, Universidad de Caldas, Pontificia Universidad Javeriana in Bogotá, Corporación Corpogen and ParqueSoft). The work was centered on metagenomics, bioinformatics and bioprospecting of microorganisms with the aim of building capacity for work using novel omic approaches and to provide the basis for value-added work on biodiversity. The center carried out a variety of different projects that reflected the interests of the researchers. The work involved studies of microbial diversity and metagenomics of ecosystems within the National Natural Park los Nevados in Colombia, bioprospecting using both microbial isolates, metagenomic libraries and metagenomic data mining, studies on microbial ecology and the development of workflows for bioinformatic analysis and new strategies for data analysis. We also carried out work on legal aspects and intellectual property rights in order to comply with existing regulations and to propose work schemes based on the researchers' interests. This particular work involved the study and analysis of microorganisms associated with endemic Espeletia plants in the Páramo ecosystem of the Park.

計畫名稱 Bioprospección, bioinformática y metagenómica en el Parque Nacional Natural los Nevados
辨識碼 Gebix4
經費來源 Colciencias Universidad y Centros participantes
研究區域描述 Espeletia plant phyllosphere
研究設計描述 The idea was to analyze microbial communities associated with endemic Espeletia plants. Communities were analyzed by massive sequencing (Ruiz-Pérez, 2016) and isolates were obtained to identify properties that enable microbial survival in these locations.

The personnel involved in the project:

研究主持人
María Mercedes Zambrano
作者
Carlos Ruiz
作者
Ivan Camilo Acosta

取樣方法

Leaf samples from various plant parts were taken, in triplicate, and transported to the lab where they were processed to recover culturable microorganisms and DNA.

研究範圍 Microbial isolates were obtained from Espeletia leaves sampled from plants located in páramo ecosystems (04°52'27'' N 75°15'51.4'' W).

方法步驟描述:

  1. Microbes were recovered from leaf surfaces by plating on solid R2A Agar (Difco) and Tryptone soy agar (TSA, Oxoid), and incubating several days at 25ºC. Strains were purified by re-streaking, characterized by growth on various media, and checked for the ability to withstand UVC and temperature changes. Strains were identified by sequence analysis of either the 16S rRNA gene or the ITS region.

收藏資料

蒐藏名稱 Cepario Corpogen
蒐藏編號 Registro Nacional de Colecciones Biológicas:140
上層採集品識別碼 CG
Specimen preservation methods Deep frozen

引用文獻

  1. Ruiz-Pérez CA, Restrepo S, Zambrano MM. 2016. Microbial and functional diversity within the phyllosphere of Espeletia species in an Andean high-mountain ecosystem. Appl Environ Microbiol 82:1807–1817 doi:10.1128/AEM.02781-15
  2. Van Wyngaarden, W., & Fandiño, M. (2002) Parque Nacional los Nevados. Un caso de selección y zonificación de áreas de conservación biológica. In Serie Investigación 4. Javergraf (ed.) Bogotá - Colombia: Pontificia Universidad Javeriana.

額外的元數據

目的 These strains were obtaine to have a collection of microorganisms from plants in the páramo ecosystems in order to study possible adaptations to harsh environmental conditions such as resistance to UV and daily temperature changes.
替代的識別碼 doi:10.15472/avygwj
9a6c17ba-58f6-4186-83a1-7ca3386deb73
https://ipt.biodiversidad.co/sib/resource?r=filosfera