Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados

Latest version published by Corporación CorpoGen on Aug 1, 2019 Corporación CorpoGen

These microorganisms were collected from the phyllosphere of Espeletia plants located in paramo ecosystems in the National Natural Park Los Nevados. The work was part of the Colombian Center of Genomics and Bioinformatics, GEBIX, a research consortium than worked on metagenomics and bioprospecting from 2008 to 2014.

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 50 records. 1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.

  • Occurrence (core)
    50
  • MeasurementOrFact 
    250

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

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Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Zambrano M M, Ruiz C, Acosta I C (2018): Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados. v1.4. Corporación CorpoGen. Dataset/Occurrence. https://doi.org/10.15472/avygwj

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is Corporación CorpoGen. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 9a6c17ba-58f6-4186-83a1-7ca3386deb73.  Corporación CorpoGen publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Colombian Biodiversity Information System.

Keywords

Phyllosphere microbiome; Espeletia phyllosphere; Occurrence; Observation

Contacts

Who created the resource:

María Mercedes Zambrano
Directora Científica
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org
Carlos Ruiz
Investigador
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org
Ivan Camilo Acosta
Investigador
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org

Who can answer questions about the resource:

María Mercedes Zambrano
Directora Científica
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org

Who filled in the metadata:

SiB Colombia
María Mercedes Zambrano
Directora Científica
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá DC CO 57-1-8050122
http://www.corpogen.org
Angela Cantillo
Investigadora
Corporación Corpogen Bogotá CO

Who else was associated with the resource:

Author
Corporación Corpogen
Corporación Corpogen Carrera 5 #66A-34 110231 Bogotá CO 18050122

Geographic Coverage

The samples were collected at a specific location within the PNN los Nevados (04°52'27'' N 75°15'51.4'' W). This park is located on the Cordillera Central in Colombia, it has altitudes between 2,600 and 5,321 m.a.s.l., and an area of approximately 58,300 hectares with diverse ecosystems (Van Wyngaarden, W., & Fandiño, M. 2002). It is located within the Departments of Caldas (Municipio de Villamaría), Risaralda (Municipios de Santa Rosa de Cabal y Pereira), Quindío (Municipio de Salento) and Tolima (Municipios de Ibagué, Anzoátegui, Santa Isabel, Murillo, Villahermosa, Casabianca y Herveo)(www.parquesnacionales.gov.co/).

Bounding Coordinates South West [4.874, -75.27], North East [4.874, -75.27]

Taxonomic Coverage

Microorganismos pertencientes a Bacteria y Eukarya, en la mayoría a nivel de género.

Domain  Bacteria,  Eukarya

Temporal Coverage

Start Date 2013-04-12

Project Data

The Colombian Center for Genomics and Bioinformatics of Extreme Environments (GeBiX) was established in 2008 and carried out research until 2014. This Center worked as a network that involved 14 research groups in 7 national institutions (Universidad Nacional de Colombia - Bogotá, Universidad del Cauca, Universidad del Valle, Universidad de Caldas, Pontificia Universidad Javeriana in Bogotá, Corporación Corpogen and ParqueSoft). The work was centered on metagenomics, bioinformatics and bioprospecting of microorganisms with the aim of building capacity for work using novel omic approaches and to provide the basis for value-added work on biodiversity. The center carried out a variety of different projects that reflected the interests of the researchers. The work involved studies of microbial diversity and metagenomics of ecosystems within the National Natural Park los Nevados in Colombia, bioprospecting using both microbial isolates, metagenomic libraries and metagenomic data mining, studies on microbial ecology and the development of workflows for bioinformatic analysis and new strategies for data analysis. We also carried out work on legal aspects and intellectual property rights in order to comply with existing regulations and to propose work schemes based on the researchers' interests. This particular work involved the study and analysis of microorganisms associated with endemic Espeletia plants in the Páramo ecosystem of the Park.

Title Bioprospección, bioinformática y metagenómica en el Parque Nacional Natural los Nevados
Identifier Gebix4
Funding Colciencias Universidad y Centros participantes
Study Area Description Espeletia plant phyllosphere
Design Description The idea was to analyze microbial communities associated with endemic Espeletia plants. Communities were analyzed by massive sequencing (Ruiz-Pérez, 2016) and isolates were obtained to identify properties that enable microbial survival in these locations.

The personnel involved in the project:

Principal Investigator
María Mercedes Zambrano
Author
Carlos Ruiz
Author
Ivan Camilo Acosta

Sampling Methods

Leaf samples from various plant parts were taken, in triplicate, and transported to the lab where they were processed to recover culturable microorganisms and DNA.

Study Extent Microbial isolates were obtained from Espeletia leaves sampled from plants located in páramo ecosystems (04°52'27'' N 75°15'51.4'' W).

Method step description:

  1. Microbes were recovered from leaf surfaces by plating on solid R2A Agar (Difco) and Tryptone soy agar (TSA, Oxoid), and incubating several days at 25ºC. Strains were purified by re-streaking, characterized by growth on various media, and checked for the ability to withstand UVC and temperature changes. Strains were identified by sequence analysis of either the 16S rRNA gene or the ITS region.

Collection Data

Collection Name Cepario Corpogen
Collection Identifier Registro Nacional de Colecciones Biológicas:140
Parent Collection Identifier CG
Specimen preservation methods Deep frozen

Bibliographic Citations

  1. Ruiz-Pérez CA, Restrepo S, Zambrano MM. 2016. Microbial and functional diversity within the phyllosphere of Espeletia species in an Andean high-mountain ecosystem. Appl Environ Microbiol 82:1807–1817 doi:10.1128/AEM.02781-15
  2. Van Wyngaarden, W., & Fandiño, M. (2002) Parque Nacional los Nevados. Un caso de selección y zonificación de áreas de conservación biológica. In Serie Investigación 4. Javergraf (ed.) Bogotá - Colombia: Pontificia Universidad Javeriana.

Additional Metadata

Purpose These strains were obtaine to have a collection of microorganisms from plants in the páramo ecosystems in order to study possible adaptations to harsh environmental conditions such as resistance to UV and daily temperature changes.
Alternative Identifiers doi:10.15472/avygwj
9a6c17ba-58f6-4186-83a1-7ca3386deb73
https://ipt.biodiversidad.co/sib/resource?r=filosfera