Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados

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How to cite

Researchers should cite this work as follows:

Zambrano M M, Ruiz C, Acosta I C (2020): Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados. v1.5. Corporación CorpoGen. Dataset/Occurrence. https://doi.org/10.15472/avygwj

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The publisher and rights holder of this work is Corporación CorpoGen. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 9a6c17ba-58f6-4186-83a1-7ca3386deb73.  Corporación CorpoGen publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Colombian Biodiversity Information System.

Keywords

Phyllosphere microbiome; Espeletia phyllosphere; Occurrence; Observation

Contacts

Geographic Coverage

The samples were collected at a specific location within the PNN los Nevados (04°52'27'' N 75°15'51.4'' W). This park is located on the Cordillera Central in Colombia, it has altitudes between 2,600 and 5,321 m.a.s.l., and an area of approximately 58,300 hectares with diverse ecosystems (Van Wyngaarden, W., & Fandiño, M. 2002). It is located within the Departments of Caldas (Municipio de Villamaría), Risaralda (Municipios de Santa Rosa de Cabal y Pereira), Quindío (Municipio de Salento) and Tolima (Municipios de Ibagué, Anzoátegui, Santa Isabel, Murillo, Villahermosa, Casabianca y Herveo)(www.parquesnacionales.gov.co/).

Taxonomic Coverage

Microorganismos pertencientes a Bacteria y Eukarya, en la mayoría a nivel de género.

Temporal Coverage

Project Data

The Colombian Center for Genomics and Bioinformatics of Extreme Environments (GeBiX) was established in 2008 and carried out research until 2014. This Center worked as a network that involved 14 research groups in 7 national institutions (Universidad Nacional de Colombia - Bogotá, Universidad del Cauca, Universidad del Valle, Universidad de Caldas, Pontificia Universidad Javeriana in Bogotá, Corporación Corpogen and ParqueSoft). The work was centered on metagenomics, bioinformatics and bioprospecting of microorganisms with the aim of building capacity for work using novel omic approaches and to provide the basis for value-added work on biodiversity. The center carried out a variety of different projects that reflected the interests of the researchers. The work involved studies of microbial diversity and metagenomics of ecosystems within the National Natural Park los Nevados in Colombia, bioprospecting using both microbial isolates, metagenomic libraries and metagenomic data mining, studies on microbial ecology and the development of workflows for bioinformatic analysis and new strategies for data analysis. We also carried out work on legal aspects and intellectual property rights in order to comply with existing regulations and to propose work schemes based on the researchers' interests. This particular work involved the study and analysis of microorganisms associated with endemic Espeletia plants in the Páramo ecosystem of the Park.

The personnel involved in the project:

Sampling Methods

Leaf samples from various plant parts were taken, in triplicate, and transported to the lab where they were processed to recover culturable microorganisms and DNA.

Method step description:

1. Microbes were recovered from leaf surfaces by plating on solid R2A Agar (Difco) and Tryptone soy agar (TSA, Oxoid), and incubating several days at 25ºC. Strains were purified by re-streaking, characterized by growth on various media, and checked for the ability to withstand UVC and temperature changes. Strains were identified by sequence analysis of either the 16S rRNA gene or the ITS region.

Collection Data

Bibliographic Citations

1. Ruiz-Pérez CA, Restrepo S, Zambrano MM. 2016. Microbial and functional diversity within the phyllosphere of Espeletia species in an Andean high-mountain ecosystem. Appl Environ Microbiol 82:1807–1817 doi:10.1128/AEM.02781-15
2. Van Wyngaarden, W., & Fandiño, M. (2002) Parque Nacional los Nevados. Un caso de selección y zonificación de áreas de conservación biológica. In Serie Investigación 4. Javergraf (ed.) Bogotá - Colombia: Pontificia Universidad Javeriana.

Additional Metadata