GBIF Integrated Publishing Toolkit(IPT)

free and open access to biodiversity data

Análisis de la variación fenotípica y diversidad genética de las poblaciones de Phytophthora spp. en diferentes hospederos en Colombia

Pathogen variation plays an important role in the dynamics of infectious diseases. In this study, the genetic variation of 279 Phytophthora infestans isolates was assessed using a combination of 12 microsatellite simple-sequence repeat markers. Isolates were collected from 11 different potato cultivars in 11 different geographic localities of the central region of Colombia. The objective of this study was to determine whether populations were differentiated by host genotype or geographic origin. Within a single clonal lineage, EC-1, 76 genotypes were detected. An analysis of molecular variance attributed most of the variation to differences within host genotypes rather than among the host genotypes, suggesting that host cultivars do not structure the populations of the pathogen. Furthermore, the lack of a genetic population structure according to the host cultivar was confirmed by all of the analyses, including the Bayesian clustering analysis and the minimum spanning network that used the Bruvo genetic distance, which suggested that there are no significant barriers to gene flow for P. infestans among potato cultivars. According to the geographic origin, the populations of P. infestans were also not structured, and most of the variation among the isolates was attributed to differences within localities. Only some but not all localities in the north and west of the central region of Colombia showed some genetic differentiation from the other regions. The absence of sexual reproduction of this pathogen in Colombia was also demonstrated. Important insights are discussed regarding the genetic population dynamics of the P. infestans populations of the central region of Colombia that were provided by the results. In Colombia, there is a high genetic variation within the EC-1 clonal lineage with closely related genotypes, none dominant, that coexist in a wide geographic area and on several potato cultivars.

Certificado en PDF
Número del certificado170E5312912
Fecha del certificado2020-03-16
Certificado en PDFdescargar (57 KB)
Contacto del recurso
NombreSilvia Restrepo
PosiciónProfesora titular
OrganizaciónUniversidad de los Andes
Dirección Cra. 1 #18a 12, Bogotá, Cundinamarca, COLOMBIA, Código postal: 111711
Contactosrestrep@uniandes.edu.co Tel: 339 49 99
Página Webhttps://uniandes.edu.co/
Contacto del permiso
NombreSilvia Restrepo
PosiciónProfesora titular
OrganizaciónUniversidad de los Andes
Dirección Cra. 1 #18a 12, Bogotá, Cundinamarca, COLOMBIA, Código postal: 111711
Contactosrestrep@uniandes.edu.co Tel: 339 49 99
Página Webhttps://uniandes.edu.co/
Proveedor de los metadatos
NombreSilvia Restrepo
PosiciónProfesora titular
OrganizaciónUniversidad de los Andes
Dirección Cra. 1 #18a 12, Bogotá, Cundinamarca, COLOMBIA, Código postal: 111711
Contactosrestrep@uniandes.edu.co Tel: 339 49 99
Página Webhttps://uniandes.edu.co/
Información del Permiso
Autoridad ambientalMinisterio de Ambiente y Desarrollo Sostenible
Número del permisoArtículo 252 de la Ley 1753 de 2015
Titular del permisoUniversidad de los Andes
Nit o cédula860.007.386-1
Fecha de emisión2015-06-09
Cobertura Geográfica
DescripciónColombia CO Putumayo Colon Colon Colombia CO Nariño Pasto Pasto Colombia CO Nariño Consaca Consaca Colombia CO Putumayo San Francisco San Francisco Colombia CO Putumayo Santiago Santiago Colombia CO Putumayo Sibundoy Sibundoy Colombia CO Nariño Buesaco Buesaco Colombia CO Nariño Iles Iles Colombia CO Antioquia Antioquia Antioquia Colombia CO Cundinamarca Sabana Occidente Sabana Occidente Colombia CO Cundinamarca Subia Subia
Coordenadas1.19, 1.207 / -77.466, -76.918 (Latitud mínima, máxima / Longitud mínima/máxima)
Cobertura Taxonómica
Descripción Muestras del género Phytophthora.
Género Phytophthora
Especie Phytophthora infestans
Cobertura Temporal
Fecha inicial / Fecha final2009-11-21 / 2015-05-10
Métodos de Muestreo
Descripción del muestreoThree to four leaves were sampled from 15 randomly selected plants that showed late blight symptoms from each field. Approximately 1 cm2 of infected leaf, between the necrotic and sporulating areas, was excised and placed in contact with a potato dextrose agar (PDA) medium that contained antibiotics (1 ml of rifampicin, 1 ml of ampicillin, 1 ml of chloramphenicol, and 1 ml of pentachlonitrobenzene per liter) (Danies et al. 2013). In total, 278 isolates were successfully isolated and subsequently transferred again into a fresh PDA medium. All plates were grown at 18C in the dark for 7 days and were stored in the Phytophthora collection at Universidad de los Andes (Bogotá, Colombia). Duplicates of all isolates collected in this study were stored in the Museum of Natural History at Universidad de los Andes.
Palabras Clave
Phytophthora Colombia Population Structure Genetic variation Host; ; Specimen