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Análisis de la variación fenotípica y diversidad genética de las poblaciones de Phytophthora spp. en diferentes hospederos en Colombia

Pathogen variation plays an important role in the dynamics of infectious diseases. In this study, the genetic variation of 279 Phytophthora infestans isolates was assessed using a combination of 12 microsatellite simple-sequence repeat markers. Isolates were collected from 11 different potato cultivars in 11 different geographic localities of the central region of Colombia. The objective of this study was to determine whether populations were differentiated by host genotype or geographic origin. Within a single clonal lineage, EC-1, 76 genotypes were detected. An analysis of molecular variance attributed most of the variation to differences within host genotypes rather than among the host genotypes, suggesting that host cultivars do not structure the populations of the pathogen. Furthermore, the lack of a genetic population structure according to the host cultivar was confirmed by all of the analyses, including the Bayesian clustering analysis and the minimum spanning network that used the Bruvo genetic distance, which suggested that there are no significant barriers to gene flow for P. infestans among potato cultivars. According to the geographic origin, the populations of P. infestans were also not structured, and most of the variation among the isolates was attributed to differences within localities. Only some but not all localities in the north and west of the central region of Colombia showed some genetic differentiation from the other regions. The absence of sexual reproduction of this pathogen in Colombia was also demonstrated. Important insights are discussed regarding the genetic population dynamics of the P. infestans populations of the central region of Colombia that were provided by the results. In Colombia, there is a high genetic variation within the EC-1 clonal lineage with closely related genotypes, none dominant, that coexist in a wide geographic area and on several potato cultivars.

Certificado en PDF
Número del certificado170CBF61E1B
Fecha del certificado2020-03-12
Certificado en PDFdescargar (58 KB)
Contacto del recurso
NombreSilvia Restrepo
PosiciónProfesora titular
OrganizaciónUniversidad de los Andes
Dirección Cra. 1 #18a 12, Bogotá, Cundinamarca, COLOMBIA, Código postal: 111711
Contactosrestrep@uniandes.edu.co Tel: 339 49 99
Página Webhttps://uniandes.edu.co/
Contacto del permiso
NombreSilvia Restrepo
PosiciónProfesora titular
OrganizaciónUniversidad de los Andes
Dirección Cra. 1 #18a 12, Bogotá, Cundinamarca, COLOMBIA, Código postal: 111711
Contactosrestrep@uniandes.edu.co Tel: 339 49 99
Página Webhttps://uniandes.edu.co/
Proveedor de los metadatos
NombreSilvia Restrepo
PosiciónProfesora titular
OrganizaciónUniversidad de los Andes
Dirección Cra. 1 #18a 12, Bogotá, Cundinamarca, COLOMBIA, Código postal: 111711
Contactosrestrep@uniandes.edu.co Tel: 339 49 99
Página Webhttps://uniandes.edu.co/
Información del Permiso
Autoridad ambientalMinisterio de Ambiente y Desarrollo Sostenible
Número del permisoArtículo 252 Ley 1753 de 2015 CONTRATO RGE256
Titular del permisoUniversidad de los Andes
Nit o cédula860.007.386-1
Fecha de emisión2015-06-09
Cobertura Geográfica
DescripciónColombia Cundinamarca Bojacá Bojacá Colombia Cundinamarca Mosquera Venecia Colombia Cundinamarca Madrid Monte Cristo Colombia Cundinamarca El Rosal Casa Blanca Colombia Cundinamarca Subachoque Vereda Guamal Colombia Cundinamarca Chocontá Vereda Tilatá Colombia Cundinamarca Villapinzón Tibita Colombia Cundinamarca Sesquilé El Uval Colombia Cundinamarca Sesquilé El Hato Colombia Cundinamarca Sesquilé Agua Blanca Colombia Cundinamarca Villapinzón Chasques Colombia Cundinamarca Villapinzón Bosavita Cundinamarca Villapinzón San Pablo Cundinamarca Villapinzón Salitre Alto Cundinamarca Villapinzón Salitre Bajo Colombia Cundinamarca Bogotá Pasquilla Colombia Cundinamarca Bogotá Pasquillita Colombia Cundinamarca Bogotá Olarte Colombia Cundinamarca Sesquile El Hato Colombia Cundinamarca Sesquile Chaleche Colombia Cundinamarca Sesquile Tierra Negra Colombia Cundinamarca Sesquile Agua Blanca Colombia Cundinamarca Sesquile Rancheria Colombia Cundinamarca Sesquile El Uval Colombia Cundinamarca Zipaquirá La florida Colombia Cundinamarca Zipaquirá Rio Frio Colombia Cundinamarca Zipaquirá El Cruce Colombia Cundinamarca Zipaquirá Paramo Guerrero Colombia Cundinamarca Tausa LlanoGrande Colombia Cundinamarca Tausa Paramo Bajo Colombia Cundinamarca Tausa Lagunita Colombia Cundinamarca Tausa Tierra Negra Colombia Cundinamarca Cogua Salitre Colombia Cundinamarca Tausa Salitre Colombia Cundinamarca Subachoque La Porquera Colombia Cundinamarca Subachoque Galdamez Colombia Cundinamarca Subachoque Cascajal Colombia Cundinamarca Subachoque El paramo Colombia Cundinamarca Subachoque El guamal
Coordenadas4.417, 5.293 / -74.287, -73.536 (Latitud mínima, máxima / Longitud mínima/máxima)
Cobertura Taxonómica
Descripción Muestras del género Phytophthora.
Género Phytophthora
Cobertura Temporal
Fecha inicial / Fecha final2016-07-07 / 2016-12-06
Métodos de Muestreo
Descripción del muestreoIn total, 1,298 putatively infected leaves that exhibited symptoms akin to late potato blight were collected between July and December 2016 from potato crops (S. tuberosum Andigenum and Phureja Groups) in the central region of Colombia (Cundinamarca region). The host cultivars that corresponded to the Phureja Group were Criolla Colombia, Criolla Dorada, Criolla Ocarina, Milagros, and Paola; and the Andigenum Group cultivars were Diacol Capiro, Parda Pastusa, Superior, Pastusa Suprema, Tuquerren ̃a, and ICA Unica. The Pastusa Suprema and Paola cultivars were considered resistant to late blight disease; the Milagros and ICA Unica cultivars were considered moderately resistant to late blight disease; and the Tuquerreña, Superior, Parda Pastusa, Diacol Capiro, Criolla Colombia, Criolla Ocarina, and Criolla Dorada were considered susceptible to late blight disease. In total, 11 geographic localities were sampled, including the north (N) of the central region, which included the localities of Tausa, Cogua, and Zipaquirá; the west (W) of the central region, which included Subachoque, El Rosal, Bojacá, and Mosquera; the central (C) part of the central region, which included Bogotá; and the northeast (NE) of the central region, which included Villapinzón, Chocontá, and Sesquilé. The classification of regions as N, W, C, and NE was based on the characteristics of the agronomic and production systems of each locality. Three to four leaves were sampled from 15 randomly selected plants that showed late blight symptoms from each field. Approximately 1 cm2 of infected leaf, between the necrotic and sporulating areas, was excised and placed in contact with a potato dextrose agar (PDA) medium that contained antibiotics (1 ml of rifampicin, 1 ml of ampicillin, 1 ml of chloramphenicol, and 1 ml of pentachlonitrobenzene per liter) (Danies et al. 2013). In total, 278 isolates were successfully isolated and subsequently transferred again into a fresh PDA medium. All plates were grown at 18C in the dark for 7 days and were stored in the Phytophthora collection at Universidad de los Andes (Bogotá, Colombia). Duplicates of all isolates collected in this study were stored in the Museum of Natural History at Universidad de los Andes.
Palabras Clave
Phytophthora Colombia Population Structure Genetic variation Host; Specimen