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Phylogeography and Sex-Biased Dispersal across Riverine Manatee Populations (Trichechus inunguis and Trichechus manatus) in South America

Phylogeographic patterns and sex-biased dispersal were studied in riverine populations of West Indian (Trichechus manatus) and Amazonian manatees (T. inunguis) in South America, using 410bp D-loop (Control Region, Mitochondrial DNA) sequences and 15 nuclear microsatellite loci. This multi-locus approach was key to disentangle complex patterns of gene flow among populations. D-loop analyses revealed population structuring among all Colombian rivers for T. manatus, while microsatellite data suggested no structure. Two main populations of T. inunguis separating the Colombian and Peruvian Amazon were supported by analysis of the D-loop and microsatellite data. Overall, we provide molecular evidence for differences in dispersal patterns between sexes, demonstrating male-biased gene flow dispersal in riverine manatees. These results are in contrast with previously reported levels of population structure shown by microsatellite data in marine manatee populations, revealing low habitat restrictions to gene flow in riverine habitats, and more significant dispersal limitations for males in marine environments.

Certificado en PDF
Número del certificado170A1C8FC27
Fecha del certificado2020-03-03
Certificado en PDFdescargar (56 KB)
Contacto del recurso
NombreSusana Caballero
PosiciónProfesora asociada
OrganizaciónUniversidad de los Andes
Dirección Cra. 1 #18a 12, Bogotá, Cundinamarca, COLOMBIA, Código postal: 111711
Contactosj.caballero26@uniandes.edu.co Tel: 339 49 99
Página Webhttps://uniandes.edu.co/
Contacto del permiso
NombreYiselle Cano
PosiciónAnalista laboratorio
OrganizaciónUniversidad de los Andes
Dirección Cra. 1 #18a 12, Bogotá, Cundinamarca, COLOMBIA, Código postal: 111711
Contactoyp.cano137@uniandes.edu.co Tel: 339 49 99
Página Webhttps://uniandes.edu.co/
Proveedor de los metadatos
NombreSusana Caballero
PosiciónProfesora asociada
OrganizaciónUniversidad de los Andes
Dirección Cra. 1 #18a 12, Bogotá, Cundinamarca, COLOMBIA, Código postal: 111711
Contactosj.caballero26@uniandes.edu.co Tel: 339 49 99
Página Webhttps://uniandes.edu.co/
Información del Permiso
Autoridad ambientalMinisterio de Ambiente y Desarrollo Sostenible
Número del permisoArtículo 252 de la Ley 1753 de 2015
Titular del permisoUniversidad de los Andes
Nit o cédula860.007.386-1
Fecha de emisión2015-06-09
Cobertura Geográfica
DescripciónColombia CO Amazonas Río Amazonas Colombia CO Cordoba Río Sinú Colombia CO Atlántico Río Magdalena Colombia CO Cordoba Río San Jorge Colombia CO Santander Ciénaga de Paredes, Río Magdalena Colombia CO Río Meta Colombia CO Vichada Río Orinoco
Coordenadas7.442, 7.442 / -73.778, -73.778 (Latitud mínima, máxima / Longitud mínima/máxima)
Cobertura Taxonómica
Descripción Manatíes de las especies Trichechus inunguis y Trichechus manatus
Especie Trichechus manatus, Trichechus inunguis
Cobertura Temporal
Fecha inicial / Fecha final2010-01-01 / 2011-01-01
Métodos de Muestreo
Descripción del muestreoSkin, blood and fecal samples were collected from wild and captive manatees. Bone, skin, and muscle samples were collected from carcasses. Skin, muscle and feces were preserved in 70% ethanol, and blood samples in EDTA lysis buffer [10 mM NaCl, 100 mM EDTA, 100 mM Tris (pH 8), and 1% (w/v) SDS]. A total of 97 samples were collected from 39 for T. inunguis and 58 for T. manatus. Amazonian manatees were sampled from four Amazon tributaries, and five specific locations: (I) Colombian Amazon River (n = 6), (II) Peruvian Amazon River (n= 7), (III) Ucayali River (n = 13), (IV) Maran ̃o ́n River (n= 9), and (V) Napo River (n = 5). West Indian manatees were sampled from six Colombian distinct locations: (I) Sinu ́ River (n = 17), (II) Northern Magdalena River (n = 19), (III) San Jorge River (n = 10), (IV) Magdalena River’s Ciénaga de Paredes (n = 7), (V) Meta River (n = 2), and (VI) Orinoco River (n = 3). Northern Magdalena River was considered a different sampling location from Magdalena River’s Ciénaga de Paredes (marsh), as it is located in the middle Magdalena basin, and is not directly connected to the Magdalena River. DNA extractions were performed using a phenol-chloroform protocol.
Palabras Clave
Trichechus Population genetics Gene flow Phylogeography; Specimen